Volume 15, Issue 6

GREEN SYNTHESIS OF ZINC OXIDE NANOPARTICLES USING Catharanthus roseus PLANT EXTRACT AND ITS ANTIMICROBIAL ACTIVITY AGAINST FOOD-BORNE PATHOGENS

Author

SUSHMITHA M; SANGEETHA A

Abstract

Green synthesis of nanoparticles using plant extracts has emerged as an eco-friendly alternative to conventional chemical methods. In the present study, zinc oxide nanoparticles (ZnO-NPs) were synthesized using aqueous leaf and flower extracts of Catharanthus roseus. The synthesized nanoparticles were characterized using UV-Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and SDS-PAGE analysis. UV-Visible analysis showed characteristic absorption peaks at 345 nm and 356 nm for leaf- and flower-mediated ZnO-NPs, respectively, confirming nanoparticle formation. FTIR analysis revealed the presence of hydroxyl, carbonyl, and alkyl functional groups involved in the reduction and stabilization of nanoparticles. SEM analysis demonstrated the formation of rod- and cuboid-shaped ZnO-NPs. SDS-PAGE analysis indicated the presence of proteinaceous biomolecules that may contribute to nanoparticle synthesis and stabilization. The antimicrobial activity of the synthesized nanoparticles was evaluated against Escherichia coli, Staphylococcus aureus, Aspergillus flavus, Aspergillus niger, and Fusarium spp. ZnO-NPs exhibited enhanced antimicrobial activity compared with crude plant extracts. Flower-derived ZnO-NPs demonstrated the highest antibacterial activity against Escherichia coli, producing 62.14% bacterial cell death at 30 µL concentration during MIC analysis, which may be attributed to reactive oxygen species generation and membrane disruption. The results indicate that Catharanthus roseus-mediated ZnO-NPs possess promising antimicrobial potential and may serve as eco-friendly nanomaterials for biomedical and food-related applications.

Keywords: Antibacterial activity, Antifungal activity, Catharanthus roseus, Green synthesis, Nanotechnology, Zinc oxide nanoparticles.

 

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DOI

https://doi.org/10.62226/ijarst20262719

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