Volume 15, Issue 5

Comprehensive Phytochemical Profiling and Gas Chromatography–Mass Spectrometry (GC–MS) Analysis of Mucuna pruriens Leaves

Author

Dr.Motilal Srivastava, Dr. Md. Sarfaraz Ahmad

Abstract

Mucuna pruriens also known as Velvet Beans is an annual climbing shrub that has been claimed traditionally to possess anti anemic potentials. Mucuna pruriens is one of the most promising wild underutilized medicinal legume belonging to family Fabaceae. It is used in ayurvedic as well as various traditional systems of medicine. This plant was widely utilized in treatment of various disorders. Also, it is a rich source of nutrients as well as used as a flavoring agent in the bakery industry. The traditional use of plants has declined due to scarcity of plant species, because of human activities and also by over grazing by animals. Therefore, it has become the need of hour to conserve these plant species. The present study was aimed to investigate leaves phytochemicals by preliminary phytochemical screening and Gas Chromatography-Mass Spectroscopy (GC-MS) analysis in five different solvents. Preliminary phytochemical screening revealed presence of alkaloids, flavonoids, phenols, tannins, saponins, glycosides, steroids and terpenoids. The chemical profiling revealed the presence of high-value phytochemicals like terpenes, alkaloids and phenolic compounds in these extracts.  GC-MS analysis revealed twenty-four and thirty bioactive compounds from the leaves and seeds respectively and it was solvent specific. GC–MS analysis led to the identification of several fatty acids, including linoleic acid, palmitic acid, oleic acid, and stearic acid, which are known for their therapeutic and nutritional benefits. These findings support the potential of M. pruriens leaves as a source of bioactive compounds and essential fatty acids, highlighting their relevance in nutraceutical and pharmaceutical applications. However, reported bioactive compounds highlight its nutritional importance and validate the use of the plant to cure various disorders by traditional practitioners.

Keywords: Mucuna pruriens, Phytochemical Screening, Gas Chromatography–Mass Spectrometry (GC–MS), Bioactive Compounds, Medicinal Plants, Nutraceutical Applications

 

DOI Link: https://doi.org/10.62226/ijarst202626700

Google Scholar: https://scholar.google.com/Comprehensive-Phytochemical-Profiling-and-Gas-Chromatography–Mass-Spectrometry-(GC–MS)-Analysis-of-Mucuna-pruriens-Leaves

References:

[1] B. P. Bhusare, M. L. Ahire, C. K. John, and T. D. Nikam, “Uraria picta: A comprehensive review on evidence of utilization and strategies of conservation,” Journal of Phytology, vol. 13, pp. 41–47, 2021.

[2] M. K. Singh, A. K. Singh, and M. S. Ahmad, “Antimicrobial and antioxidant potential of Achyranthes aspera Linn. and its increased prevalence in cosmetics,” Indian Journal of Applied & Pure Biology, vol. 35, no. 2, pp. 243–254, 2020.

[3] B. P. Bhusare, C. K. John, V. P. Bhatt, and T. D. Nikam, “Colchicine induces tetraploids in in vitro cultures of Digitalis lanata Ehrh.: Enhanced production of biomass and cardiac glycosides,” Industrial Crops and Products, vol. 174, Art. no. 114167, 2021.

[4] M. K. Singh and M. S. Ahmad, “Phytochemical profile of Phyllanthus niruri L. and evaluation of its potent bioactive compounds,” Bioscience Biotechnology Research Communications, vol. 13, no. 3, pp. 1545–1551, 2020.

[5] K. H. Bindu, S. Sujatha, and S. P. Tanuja, “Genotypic variability in yield, L-Dopa and mineral nutrient profile in velvet bean (Mucuna pruriens L.) production systems,” Journal of Food Composition and Analysis, vol. 118, Art. no. 105152, 2023.

[6] J. B. Devhade, S. S. Kalwaghe, and M. J. Devade, “Mucuna nivea DC., a member of Fabaceae: Preliminary phytochemical analysis,” The Pharma Innovation Journal, vol. 4, pp. 96–98, 2015.

[7] M. S. Ahmad, “Documentation and folklore use of medicinal plants of Hathwa (Gopalganj), Bihar,” The Biobrio: An International Quarterly Journal of Life Sciences, vol. 3, no. 1–2, pp. 153–160, 2016.

[8] J. D. Hooker, The Flora of British India, vol. 5. London, U.K.: L. Reeve, 1890.

[9] F. J. Luizão, “Ciclos de nutrientes na Amazônia: respostas às mudanças ambientais e climáticas,” Ciência & Cultura, vol. 59, no. 3, pp. 31–36, 2007.

[10] M. Narayanan et al., “RETRACTED: Hepato and nephroprotective activity of methanol extract of Hygrophila spinosa and its antibacterial potential against multidrug resistant Pandoraea sputorum,” 2021.

[11] D. B. Shelke, S. Tayade, P. Gawande, and H. B. Sonawane, “GC–MS analysis and antioxidant potential of wild underutilized medicinally important legume, velvet bean (Mucuna pruriens),” Notulae Scientia Biologicae, vol. 14, no. 1, Art. no. 11098, 2022.

[12] P. Siddhuraju, K. Vijayakumari, and K. Janardhanan, “Chemical composition and protein quality of the little-known legume, velvet bean (Mucuna pruriens (L.) DC.),” Journal of Agricultural and Food Chemistry, vol. 44, pp. 2636–2641, 1996.

[13] A. D. Simmons, “Parkinson’s disease,” in Integrative Medicine, 4th ed., D. Rakel, Ed. Elsevier, 2018, pp. 143–151.

[14] M. Simsek and K. Whitney, “Examination of primary and secondary metabolites associated with a plant-based diet and their impact on human health,” Foods, vol. 13, no. 7, Art. no. 1020, 2024.

[15] D. Sruthi, A. Jagannathan, A. B. Chandran, H. Y. Rao, and C. Jayabaskaran, “Chromatography–mass spectrometry-based chemical profiling of Mucuna pruriens (L.) DC. and its beneficial effect against hydrogen peroxide-induced oxidative stress in HEK 293T cells and breast cancer cells,” South African Journal of Botany, vol. 159, pp. 85–97, 2023.

[16] R. L. Tavares, A. S. Silva, A. R. N. Campos, A. R. P. Schuler, and J. de S. Aquino, “Nutritional composition, phytochemicals and microbiological quality of the legume, Mucuna pruriens,” African Journal of Biotechnology, vol. 14, no. 8, p. 676, 2015.

[17] P. Tiwari, B. Kumar, M. Kaur, G. Kaur, and H. Kaur, “Phytochemical screening and extraction: A review,” Internationale Pharmaceutica Sciencia, vol. 1, pp. 98–106, 2011.

[18] K. M. G. Frota, R. A. M. Soares, and J. A. G. Arêas, “Composição química do feijão-caupi (Vigna unguiculata L. Walp), cultivar BRS-Milênio,” Ciência e Tecnologia de Alimentos, vol. 28, no. 2, pp. 470–476, 2008.

[19] C. A. Martin et al., “Ácidos graxos poli-insaturados ômega-3 e ômega-6: importância e ocorrência em alimentos,” Revista de Nutrição, vol. 19, no. 6, pp. 761–770, 2006.

DOI

https://doi.org/10.62226/IJARST202626700

PAGES : 2028-2033 | 6 VIEWS | 2 DOWNLOADS


Download Full Article

Dr.Motilal Srivastava, Dr. Md. Sarfaraz Ahmad | Comprehensive Phytochemical Profiling and Gas Chromatography–Mass Spectrometry (GC–MS) Analysis of Mucuna pruriens Leaves | DOI : https://doi.org/10.62226/IJARST202626700

Journal Frequency: ISSN 2320-1126, Monthly
Paper Submission: Throughout the month
Acceptance Notification: Within 6 days
Subject Areas: Engineering, Science & Technology
Publishing Model: Open Access
Publication Fee: USD 60  USD 50
Publication Impact Factor: 6.76
Certificate Delivery: Digital

Publish your research with IJARST and engage with global scientific minds